Limnology and Oceanography

The use of DAPI for identifying and counting aquatic microflora1

Journal article · 1980 · Cited by 5,064

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Abstract

A highly specific and sensitive fluorescing DNA stain, 4′6‐diamidino‐2‐phenylindole (DAPI) was compared with acridine orange (AO) for counting aquatic microflora. Use of DAPI improved visualization and counting of <1‐ µ m bacteria and blue‐green algae in seston‐rich samples and extended sample storage to at least 24 weeks.

DOI: 10.4319/lo.1980.25.5.0943 · Publisher: Wiley

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